| Confocal epifluorescence detection for microspheres delivered on disposable microfluidic chip |
| |
| 作者姓名： | 胡宏华 侯西云 杨国光 |
| |
| 作者单位： | State Key Laboratory of Modern Optical Instrumentation Zhejiang University Hangzhou 310027，State Key Laboratory of Modern Optical Instrumentation，Zhejiang University，Hangzhou 310027，State Key Laboratory of Modern Optical Instrumentation，Zhejiang University，Hangzhou 310027 |
| |
| 基金项目： | This work was funded by the Microsystem Center of Zhejiang University. |
| |
| 摘 要： | The laser induced fluorescence (LIF) detection system for 5-μm microspheres delivered on microfluidic chip is presented employing confocal optical scheme. The parameters of the optical system are specifically optimized for single microsphere detection. With the excitation laser spot size of 4.6 μm and optical sectioning power of 27 μm, the lowest concentration detection limit is 0.45 nmol/L, corresponding to only 122 molecules in probe volume. The microsphere detection is carried on successfully with the maximum signal-to-noise ratio (SNR) of 55.7, which provides good detection sensitivity.
|
| 本文献已被 CNKI 等数据库收录！ |
