Differential interactions of cyclodextrins with hydrophobic derivatives of sepharose CL-4B |
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Authors: | Masanobu Janado Yuki Yano Miyuki Umura Yôtaro Kondo |
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Institution: | (1) Department of Food Science, Kyoto Women's University, Higashiyama-ku, 605 Kyoto, Japan;(2) Joetsu University of Education, 943 Joetsu-shi, Nigata-ken, Japan |
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Abstract: | The partition coefficients Kav between the solution phase and octyl- and phenyl-sepharose CL-4B were determined for three cyclodextrins (CDs), -CD, -CD and -CD in various aqueous solutions, as a measure of their interactions with the two hydrophobic ligands. Kav of the CDs increased in the order of -CD< -CD< -CD for octyl-sepharose CL-4B and -CD< -CD< -CD for phenyl-sepharose CL-4B. In all cases, Kav increased by increasing NaCl concentration in the aqueous solution phase and also by lowering temperature, but in the presence of NaBr and NaSCN, both chaotropic salts, Kav decreased markedly. The spontaneity of the transfer of the CDs from the aqueous solution phases to the gel phases was due to the enthalpy decrease. It was shown that discrete separation of the three CDs can be achieved by the hydrophobic chromatography on a short column (1×25cm) of octyl-sepharose CL-4B by adjusting the NaCl concentration and temperature. |
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Keywords: | Cyclodextrins host-guest interaction octyl- and phenyl-sepharose hydrophobic chromatography thermodynamic parameters salt effect |
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