Ligand accessibility to receptor binding sites enhanced by movable polyrotaxanes |
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Authors: | Hyun Hoon Yui Nobuhiko |
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Affiliation: | School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa, Japan. |
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Abstract: | Functionalized polyrotaxanes are utilized to investigate the relation to multivalent interactions between the mannose moiety and Con A immobilized surfaces. According to the results of SPR spectroscopy, the mannose-conjugated polyrotaxanes show a higher response than any other mannose conjugate on both surfaces of high- and low-density Con A. Moreover, the results of the FRET analysis suggest that the mobility of α-cyclodextrins in the polyrotaxane more efficiently contributes to their binding interactions in a multivalent manner. This well-defined polyrotaxane system provides control over ligand density, ligand mobility, and gives an efficient response to the biological interaction receptor, which has not been easy to achieve in covalently bound polymeric systems. |
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Keywords: | concanavalin A molecular recognition multivalent interactions polyrotaxanes surface plasmon resonance |
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