| 假丝酵母类金属硫蛋白的分离、纯化及功能研究 |
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| 引用本文： | 李明春,杨炤,张飙,邢来君.假丝酵母类金属硫蛋白的分离、纯化及功能研究[J].南开大学学报,2003,36(3):123-128. |
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| 作者姓名： | 李明春 杨炤 张飙 邢来君 |
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| 作者单位： | [1]南开大学微生物学系，天津300071 [2]天津中医学院，天津300193 |
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| 摘 要： | 假丝酵母Cu-12(Candida sp．Cu-12)经Cu^2 诱导产生的蛋白进行Sephadex G-100和纤维素DE-52分离纯化及理化性质测定，获得的蛋白具有四个亚型，其主理化性质均与MT的定义相符，因此确定得到了假丝酵母的类金属硫蛋白，同时测定了该Cu—MT清除羟自由基和氧自由基的能力．
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| 关 键 词： | 类金属硫蛋白 假丝酵母 分离纯化 自由基 |
| 文章编号： | 0465-7942(2003)03-0123-06 |
| 修稿时间： | 2002年12月27 |
STUDY ON ISOLATION, PURIFICATION AND FUNCTION OF METALLOTHIONEIN-LIKE PROTEIN FROM CANDIDA SP. |
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| LI Mingchun,YANG Zhao,ZHANG Biao,XING Laijun.STUDY ON ISOLATION, PURIFICATION AND FUNCTION OF METALLOTHIONEIN-LIKE PROTEIN FROM CANDIDA SP.[J].Acta Scientiarum Naturalium University Nankaiensis,2003,36(3):123-128. |
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| Authors: | LI Mingchun YANG Zhao ZHANG Biao XING Laijun |
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| Abstract: | The protein was produced from Candida sp. Cu-12 induced with CuCl2, isolated by means of Sephadex G-100 and DEAE-cellulosecolumn chroma tography, and determined physicochemical characteristics. Four types of this protein were found and most physicochemi-cal characteristics were in line with the definition of metallothionein. So those proteins were metallothionein-like protein of Candida sp. Meanwhile, the ability of Cu-MTs to scavenge hy-droxyl radical and oxygen radical was determined. |
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| Keywords: | Metallothionein-Hke proteins Candida sp isolate and purify radical |
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