Ultrasound assisted combined molecularly imprinted polymer for selective extraction of nicotinamide in human urine and milk samples: Spectrophotometric determination and optimization study |
| |
| Institution: | 1. Institute of Pharmacy, Bundelkhand University, Jhansi, Uttar Pradesh, India;2. Department of Pharmaceutical Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences (deemed to be University), Allahabad, Uttar Pradesh, India;1. Departmentof Chemistry, Yasouj University, Yasouj 75918-74831, Iran;2. Department of Pharmacology Toxicology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran;3. Department of Chemical Engineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India;1. Department of Pharmacy, Faculty of Engineering and Technology,Annamalai University, Annamalai Nagar, Tamilnadu, India-608002;2. Malla Reddy Pharmacy College, Maisammaguda, Dhulapally, Hyderabad, India-500100;1. Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran;2. Department of Physics, Yasouj University, Yasouj 75918-74831, Iran;3. Department of Semiconductors, Materials and Energy Research Center (MERC), Karaj 3177983634, Iran;4. Department of Chemistry, Kerman Branch, Islamic Azad University, Kerman, Iran;1. Faculty of Science, Department of Chemistry, Hacettepe University, Ankara, Turkey;2. Advanced Technologies Application and Research Center, Hacettepe University, Ankara, Turkey;3. Faculty of Science and Arts, Department of Chemistry, Sinop University, Sinop, Turkey |
| |
| Abstract: | Ultrasound-assisted dispersive solid phase microextraction followed by UV–vis spectrophotometer (UA-DSPME-UV–vis) was designed for extraction and preconcentration of nicotinamide (vitamin B3) by HKUST-1 metal organic framework (MOF) based molecularly imprinted polymer (MIP). This new material was characterized by FTIR and FE-SEM techniques. The preliminary Plackett–Burman design was used for screening and subsequently the central composite design justifies significant terms and possible construction of mathematical equation which give the individual and cooperative contribution of variables like HKUST-1-MOF-NA-MIP mass, sonication time, temperature, eluent volume, pH and vortex time. Accordingly the optimum condition was set as: 2.0 mg HKUST-1-MOF-NA-MIP, 200 μL eluent and 5.0 min sonication time in center points other variables were determined as the best conditions to reach the maximum recovery of the analyte. The UA-DSPME-UV–vis method performances like excellent linearity (LR), limits of detection (LOD), limits of quantification of 10–5000 μg L−1 with R2 of 0.99, LOD (1.96 ng mL−1), LOQ (6.53 μg L−1), respectively show successful and accurate applicability of the present method for monitoring analytes with within- and between-day precision of 0.96–3.38%. The average absolute recoveries of the nicotinamide extracted from the urine, milk and water samples were 95.85–101.27%. |
| |
| Keywords: | Experimental design Human urine Molecularly imprinted polymer Nicotinamide DSPME-UV–Vis Milk samples Ultrasound assisted |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
