| DNA的荧光分析--DNA对吖啶橙(AO)-中性红(NR)能量转移影响的研究 |
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| 引用本文： | 陈鸿琪,夏闽.DNA的荧光分析--DNA对吖啶橙(AO)-中性红(NR)能量转移影响的研究[J].曲阜师范大学学报,2000,26(4). |
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| 作者姓名： | 陈鸿琪 夏闽 |
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| 作者单位： | 临沂师范学院科研处!276001，山东省临沂市，临沂师范学院科研处!276001，山东省临沂市 |
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| 摘 要： | 介绍了近几年荧光能量转移体系用于DNA分析测定方法的进展 .通过实验对吖啶橙 (AO)_中性红 (NR)荧光体系能量转移进行分析 ,说明使用该体系可用于对DNA定量测定 .然后通过Forster能量转移理论 ,计算DNA加入前后AO_NR体系临界距离变化 ,从某种程度上说明DNA对AO_NR荧光淬灭的影响 .
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| 关 键 词： | 荧光能量转移 荧光淬灭 吖啶橙(AO)-中性红(NR) 荧光探针 临界距离 |
FLUORESCENT ANALYSIS OF DNA -RESEARCH OF DNA ON ACRIDINE ORANGE(AO)-NEUTRAL RED(NR) ENERGY TRANSSFER |
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| CHEN Hong-qi,XIA Min.FLUORESCENT ANALYSIS OF DNA -RESEARCH OF DNA ON ACRIDINE ORANGE(AO)-NEUTRAL RED(NR) ENERGY TRANSSFER[J].Journal of Qufu Normal University(Natural Science),2000,26(4). |
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| Authors: | CHEN Hong-qi XIA Min |
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| Abstract: | This paper introduces recent progress in method of applying fluorescence energy transfer system for the determination of DNA. The energy transfer of Acridine Orange (AO) Neutral Red(NR) system is invested by our experiments, in order to prove the possibility of quantatively testing DNA with the fluorescene probe of this system. At the same time, Critical ranges of AO NR, before and after the addition of DNA, are calculated respectively, according to the Forster theory of energy transfer. Thus, to some extent, we illustrate the influence of DNA on AO NR fluoresence quenching. |
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| Keywords: | fluorescene energy transfer fluorescenes quenching acridine orange(AO) Neutral red(NR) Critical range fluoresence probe |
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