SOLUTION PROPERTIES OF ANTITUMOR CARBOXYMETHYLATED DERIVATIVES OF α-(1→3)-D-GLUCAN FROM GANODERMA LUCIDUM |
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作者姓名: | Li-na Zhang Mei Zhang Jing-hua Chen Fan-bo Zeng |
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作者单位: | Li-na Zhang;Mei Zhang;Jing-hua Chen;Fan-bo Zeng Department of Chemistry Wuhan University,Wuhan 430072,China Pharmacy College,Tongji Medical University,Wuhan 430072,China |
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基金项目: | This work was financially supported by the Research Fund for the Doctoral program of Higher Education (RFDP) and the National Natural Science Foundation of China (No. 20074025) |
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摘 要: | Fractions of a water insoluble α-(1→3)-D-glucan (GL) extracted from Ganoderma lucidum were carboxy-methylated (CM) to obtain water-soluble carboxymethylated derivatives (CM-GL) having a degree of substitution (DS) of0.38~0.51. Weight-average molecular weight M_w and intrinsic viscosity η] of the samples CM-GL were measured by gelpermeation chromatography combined with laser light scattering (GPC-LLS) and viscometry. The CM-GL exhibits a stifferchain in aqueous solution at 25℃ than the original glucan, The antitumor activities against Ehrlich ascites carcinoma (EAC,5×10~6) of the carboxymethylated derivatives from the α-glucan and curdlan, a β-glucan, are significantly higher than thoseof the original glucans. The effects of the relatively low molecular weight, expanded chains and better water-solubility of theCM-GL on the enhancement of antitumor activity could not be neglected. The chain stiffness decreased speedily withincrease of temperature from 40 to 60℃ or NaOH concentration from 0.1 to 0.4 in the solution, respectively, and the changeof the chain stiffness is reversible.
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关 键 词: | Solution properties Antitumor activity Carboxymethylated derivative α-D-glucan Ganoderma lucidum |
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