| Agrobacterium tumefaciens CAN TRANSFORM Triticum aestivum AND Hordeum vulgare OF GRAMINEAE |
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| 作者姓名： | 邓万银 林晓影 邵启全 |
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| 作者单位： | Institute of Genetics，Academia Sinica，Beijing 100012，PRC，Institute of Genetics，Academia Sinica，Beijing 100012，PRC，Institute of Genetics，Academia Sinica，Beijing 100012，PRC |
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| 摘 要： | So far there is no suitable vector system for the genetic engineering of monocotyledonous plants, especially cereal crops. It is first reported in this paper that Agrobacterium tumefaciens strains T37, A208 and B6 can transform some varieties of Triticum aestivum and Hordeum vulgare to form swellings and tumors. Itis shown in the experiments that phenolic compound acetosyringone may promote the transformation process. In addition, inoculating agrobacteria on appropriate plant tissues is also proved to be the key step in achieving successful transformation. The host range of A. tumefaciens and the possibility of utilizing Ti plasmid as the vector for the gene transfer of cereal crops are discussed.
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Agrobacterium tumefaciens CAN TRANSFORM Triticum aestivum AND Hordeum vulgare OF GRAMINEAE |
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| DENG WAN-YIN LIN XIAO-YING AND SHAO QI-QUAN.Agrobacterium tumefaciens CAN TRANSFORM Triticum aestivum AND Hordeum vulgare OF GRAMINEAE[J].Science in China(Chemistry),1990(1). |
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| Authors: | DENG WAN-YIN LIN XIAO-YING AND SHAO QI-QUAN |
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| Abstract: | So far there is no suitable vector system for the genetic engineering of monocotyledonous plants, especially cereal crops. It is first reported in this paper that Agrobacterium tumefaciens strains T37, A208 and B6 can transform some varieties of Triticum aestivum and Hordeum vulgare to form swellings and tumors. Itis shown in the experiments that phenolic compound acetosyringone may promote the transformation process. In addition, inoculating agrobacteria on appropriate plant tissues is also proved to be the key step in achieving successful transformation. The host range of A. tumefaciens and the possibility of utilizing Ti plasmid as the vector for the gene transfer of cereal crops are discussed. |
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| Keywords: | Agrobacterium tumefaciens Triticum aestivum Hordeum vulgarz acetosyringone transformation of cereal crops |
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