| 酸敏性PEG载体的合成及其在多肽合成中的应用 |
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| 引用本文： | 娄素君 向新. 酸敏性PEG载体的合成及其在多肽合成中的应用[J]. 离子交换与吸附, 1999, 15(1): 15-22 |
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| 作者姓名： | 娄素君 向新 |
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| 作者单位： | 南开大学化学系,吸附分离功能高分子材料国家重点实验室 |
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| 基金项目： | 国家自然科学基金!29474161 |
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| 摘 要： | 本文通过聚苯乙烯固载聚乙二醇氨基树脂与羟甲基苯氧乙酸缩合制备了具有酸敏手臂结构的对羟甲基苯氧乙酰胺ＰＥＧ树脂。在合成中改进了ＰＳ－ＰＥＧＮＨ２树脂的制备方法，提高了树脂上功能基的转化率，不必进行封闭剩余活性基的操作，就得到具有单一功能基的树脂。
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| 关 键 词： | 酸敏性 载体 PEG树脂 多肽 固相合成 |
SYNTHESIS AND APPLICATION OF A NEW ACID-LABILE POLYETHYLENE GLYCOL RESIN FOR SOLID PHASE PEPTIDE SYNTHESIS |
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| Lou Sujun, Xiang Xin, Huang Hai, Liang Xun. SYNTHESIS AND APPLICATION OF A NEW ACID-LABILE POLYETHYLENE GLYCOL RESIN FOR SOLID PHASE PEPTIDE SYNTHESIS[J]. Ion Exchange and Adsorption, 1999, 15(1): 15-22 |
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| Authors: | Lou Sujun Xiang Xin Huang Hai Liang Xun |
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| Abstract: | PEG resins with acid-labile linkage, 4-hydroxymethylphenoxyacetamide were prepared by condensation of PS-PEG-NH2 and 4-hydroxymethylpheoxy acetic acid in the presence of HOBt/CC. This PS-PEG-HMPA resin derivative was applied as a solid support for the stepwise synthesis of peptide fragment of albumin KQTALYYC according to the Fmoc solid chase synthesis strategy. The coupling and the deprotection steps in peptide synthesis proceeded in nearly quantitative yields. The peptidyl-resin was cleaved by 95% TFA. The crude peptide was purified by C18 FLC and HPLC. Analysis showed that the peptide contains expected amino acid composition. The experimental results showed that condensation reaction on the PS-PEG-AMPA proceed faster than that on acid-sensitive PS resin. |
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| Keywords: | Acid-labile linkage Polyethylene glycol resin SPPS Fmoc chemistry |
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