Electrochemical cleavage of DNA in the presence of copper-sulfosalicylic acid complex |
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Authors: | Wang Yan-Ling Liu Yun-Chun Yang Zhou-Sheng Zhao Guang-Chao |
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Institution: | School of Chemistry and Materials Science, Anhui Normal University, Wuhu 24100, PR China. |
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Abstract: | Electrochemical cleavage of DNA in the presence of copper-sulfosalicylic acid Cu(ssal)(2)(2+)] complex was studied. The cleavage was observed in a certain potential region where redox cycling of Cu(ssal)(2)(2+)/Cu(ssal)(2)(+) took place. Cu(ssal)(2)(2+) complex mediate generation of reactive oxygen species from O(2) by the Fenton reaction, these radicals are capable of damaging DNA. The cleaved DNA fragments were separated by high-performance liquid chromatography (HPLC). The experimental results indicated that the method for electrochemical cleavage of DNA by Cu(ssal)(2)(2+) complex was simple and efficient. |
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