表面解吸常压化学电离质谱快速分析六味地黄丸

采用新型表面解吸常压化学电离(Surface Desorption Atmospheric Pressure Chemical Ionization, SDAPCI)质谱法, 在敞开环境下, 对潮湿的空气进行电晕放电产生试剂离子, 进而在六味地黄丸表面发生解吸电离过程, 在无需复杂预处理的前提下对六味地黄丸中的待测物进行离子化, 从而获得了六味地黄丸在正负离子模式下的化学指纹图谱, 并利用主成分分析法对质谱指纹数据进行处理, 可对6个厂家生产的多个批次产品进行较好的区分. 结果表明, SDAPCI-MS技术能够快速测定六味地黄丸的剂型和生产厂家信息, 并能够对目标组分做多级串联质谱鉴定, 发现痕量目标组分. 研究方法可望应用于中成药药品生产质量控制和成品检测等领域.

Rapid Analysis of Liuwei Dihuang Pills Using Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry

A surface desorption atomospheric pressure chemical ionization mass spectrometry (SDAPCI-MS) method was developed to obtain the fingerprint of Liuwei Dihuang pills (LDP) with minimal sample pre-treatment. In the open environment, humid air was corona discharged to produce reagent ions for desorption ionization of the analytes on the surface of the LDPs. Then the analyte ions were guided into the ion trap mass analyzer of the LTQ instrument for mass analysis. Identification of the components of interests such as gallic acid, paeonol and ursolic acid in Liuwei Dihuang pills were demonstrated by tandem mass spectrometry (MS/MS). Principal component analysis (PCA) of the mass spectral fingerprint data was used to differentiate the samples from four manufacturers. The results show that this method is a useful analytical tool for quality control in pharmaceutical industry, particularly for the traditional Chinese medicine production.