Identification of a set of genes involved in the formation of the substrate for the incorporation of the unusual "glycolate" chain extension unit in ansamitocin biosynthesis |
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Authors: | Carroll Brian J Moss Steven J Bai Linquan Kato Yasuo Toelzer Sabine Yu Tin-Wein Floss Heinz G |
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Institution: | Department of Chemistry, Box 351700, University of Washington, Seattle, Washington 98195-1700, USA. |
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Abstract: | The unusual "glycolate" extender unit at C-9/C-10 of ansamitocin is not derived from 2-hydroxymalonyl-CoA or 2-methoxymalonyl-CoA, as demonstrated by feeding experiments with the corresponding 1-13C-labeled N-acetylcysteamine thioesters but is formed from an acyl carrier protein (ACP)-bound substrate, possibly 2-methoxymalonyl-ACP, elaborated by enzymes encoded by a subcluster of five genes, asm12-17, from the ansamitocin bisosynthetic gene cluster. |
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