一种新型超临界流体萃取-高效液相色谱在线联用系统的构建

天然产物研究一直是植物学、化学和药学的重要研究领域．通过从天然产物中寻找生物活性成分和先导物是创制新药的有效途径之一．有效成分的提取是天然产物研究中最基本和最关键的环节．超临界流体萃取（Supercritical fluid extraction，简称SFE）是近年来发展较快的一种新型样品提取技术．超临界CO2作为最常用的萃取剂已被用于天然药物中非极性和弱极性有效成分的提取，尤其是挥发性和热敏性的物质．此外，通过加入适当的添加剂还可有效地萃取极性化合物，和传统的化学方法相比，

Establishment of a New On-line Coupled Supercritical Fluid Extraction(SFE) with High Performance Liquid Chromatography(HPLC) System

A new on-line coupled supercritical fluid extraction(SFE) with high performance liquid chromatography(HPLC) system was developed.Two three-port valves were used as an interface to connect SFE and HPLC.In this design,the two important processes including sample preparation and sample analysis were(integrated) into a system.Compared with off-line system,the on-line system has many advantages,such as high sensitivity,short analysis time,avoiding sample pollution,easily operation and ease to automation etc.Its application in the extraction and analysis of triterpenoids to the fruiting bodies of Ganoderma Lucidum was also described.Under the optimized extraction conditions,the similar chromatogram of the extract with on-line SFE- HPLC system was observed as well as that with off-line SFE followed by HPLC.The result showed the great potential of the on-line system in the extraction and analysis of tradition Chinese medicine.