Overexpression in Catharanthus roseus hairy roots of a truncated hamster 3-hydroxy-3-methylglutaryl-CoA reductase gene |
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| Authors: | Teresa Ayora-Talavera Joseph Chappell Edmundo Lozoya-Gloria Victor M. Loyola-Vargas |
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| Affiliation: | (1) Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Calle 43, No. 130 Col. Chuburna de Hidalgo, C.P. 97200 Mérida Yucatán, México;(2) Plant Physiology/Biochemistry/Molecular Biology Program, Agronomy Department, University of Kentucky, 40546-0091 Lexington, KY;(3) Departmento de Ingeniería Genética, CINVESTAV-IPN, Unidad Irapuato, Km 9.6 Libramiento Norte, Carretera Irapuato-León, Apdo. Postal 629, CP 36500 Irapuato, Gto., México;(4) Departmento de Biotecnología y Bioingeniería, CINVESTAV-IPN, Unidad México, Avenida Instituto Politécnico Nacional 2508 Col. San Pedro Zacatenco, CP 07360, DF, México |
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| Abstract: | Catharanthus roseus (L.) G. Don hairy roots harboring hamster 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) (EC 1.1.1.88) cDNA without membrane-binding domain were evaluated by quantifying the levels of sterols and some indol-alkaloids. Clone 236, with the highest hybridization signal, had the lowest soluble and microsomal HMGR activity and produced more ajmalicine and catharanthine than the control but had reduced campesterol concentration. Clone 19, with low hybridization signal, had high soluble HMGR activity and produced high levels of campesterol and five to seven times more serpentine than the control but a low level of ajmalicine and no accumulation of catharanthine. These results suggest a possible role for HMGR in indole alkaloid biosynthesis and a possible cosuppression of both the endogenous and foreign HMGR genes in clone 236. |
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| Keywords: | Alkaloids Catharanthus roseus hairy roots 3-hydroxy-3-methylglutaryl-CoA reductase |
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