|
|||||
|
|
A simple kinetic model of antigen-antibody reactions in particle-enhanced light scattering immunoassays |
|
| Authors: | M. Quesada,J. Puig,J.M. Delgado,J.M. Peula,J.A. Molina,R. Hidalgo-Á lvarez |
| Affiliation: | a Biocolloid and Fluid Physics Group, Department of Applied Physics, Faculty of Sciences, University of Granada, Campus Fuentenueva, Granada 18071, Spain b Research and Development Department of Biokit, Can Xicota, 08186 Lliçà d'Amunt, Barcelona, Spain c Department of Applied Physics II, University of Málaga, Málaga 29013, Spain |
| Abstract: | Polymer colloids coated by antibodies are used in diagnostic tests for the detection of antigens in biological fluids. We present in this paper a simple kinetic model for the optical monitoring of the formation of specific complexes between antigen and antibody with amplification by latex beads. The antibodies are physical or chemically adsorbed onto sulphonate polystyrene or chloromethylstyrene particles respectively. This is a very simple model combining the La Mer idea of optimum surface coverage with Langmuir adsorption of antigen molecules. The kinetic model explains reasonably well the optical response of immunolatex prepared by both methods of immobilization of antibodies on polymer carriers. According to the results obtained with this model the percentage of active IgG on latex particles is extremely low, with a maximum of a 5%. |
| Keywords: | Aggregation kinetics Antibody Covalent coupling Latex agglutination assays Polymer colloids |
| 本文献已被 ScienceDirect 等数据库收录！ | |