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| 亚硝酸钠化高铁血红蛋白的拉曼光谱量化检测研究 | |
| 引用本文： | 郭世珺,曾常春,李丽君,聂广,刘颂豪. 亚硝酸钠化高铁血红蛋白的拉曼光谱量化检测研究[J]. 光谱学与光谱分析, 2013, 33(7): 1805-1809. DOI: 10.3964/j.issn.1000-0593(2013)07-1805-05 |
| 作者姓名： | 郭世珺 曾常春 李丽君 聂广 刘颂豪 |
| 作者单位： | 1. 华南师范大学生物光子学研究院，激光生命科学教育部重点实验室，广东 广州 510631 2. 桂林医学院基础医学院，广西 桂林 541004 3. 深圳市第三人民医院，广东 深圳 518112 4. 华南师范大学信息光电子科技学院，广东 广州 510631 |
| 基金项目： | 国家自然科学基金项目，广东省科技计划项目 |
| 摘 要： | 对亚硝酸钠化的高铁血红蛋白进行了拉曼光谱特性的研究。实验中将正常血红蛋白用亚硝酸钠氧化为高铁血红蛋白，通过测定不同比例高铁血红蛋白/总血红蛋白的拉曼光谱变化研究高铁血红蛋白的拉曼光谱特异性改变，并对不同比例高铁血红蛋白/总血红蛋白在1 586，1 605和1 637 cm-1处的拉曼强度进行线性拟合，以实现其定量检测。结果显示，亚硝酸钠与血红蛋白的摩尔比为3.5∶1时可以获得完全氧化的高铁血红蛋白，其拉曼特征峰在499，1 340，1 562，1 622 cm-1附近;不同比例高铁血红蛋白/总血红蛋白在1 586，1 605和1 637 cm-1处拉曼强度的线性拟合相关系数R2分别为0.972 84，0.997 97和0.991 26，显示出很好的线性关系。研究表明，与正常血红蛋白相比，亚硝酸钠化高铁血红蛋白的拉曼光谱具有特异性改变，其拉曼特征峰的位置和强度随着高铁血红蛋白/总血红蛋白比例的变化而相应发生改变，且比值与拉曼强度存在着线性相关关系，为临床上高铁血红蛋白血症的拉曼光谱检测与量化研究提供了理论基础。 |
| 关 键 词： | 血红蛋白 高铁血红蛋白 拉曼光谱 亚硝酸钠 高铁血红蛋白血症 |
| 收稿时间： | 2012-11-14 |
Quantization Determination Study of Micro-Raman Spectroscopy of Methemoglobin Induced by Sodium Nitrite |
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| GUO Shi-jun , ZENG Chang-chun , LI Li-jun , NIE Guang , LIU Song-hao. Quantization Determination Study of Micro-Raman Spectroscopy of Methemoglobin Induced by Sodium Nitrite[J]. Spectroscopy and Spectral Analysis, 2013, 33(7): 1805-1809. DOI: 10.3964/j.issn.1000-0593(2013)07-1805-05 | |
| Authors: | GUO Shi-jun ZENG Chang-chun LI Li-jun NIE Guang LIU Song-hao |
| Affiliation: | 1. MOE Key Laboratory of Laser Life Science & Laboratory of Photonic Chinese Medicine, College of Biophotonics, South China Normal University, Guangzhou 510631, China2. School of Basic Medical Sciences, Guilin Medical University, Guilin 541004, China3. Department of Internal Medicine, Shenzhen Third People’s Hospital, Shenzhen 518112, China4. School for Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou 510631, China |
| Abstract: | In the present study, Raman spectral characteristics of methemoglobin (MetHb) induced by sodium nitrite (NaNO2) were investigated. Hemoglobin (Hb) was oxidated to MetHb with NaNO2, the Raman spectral specific changes of MetHb was studied by determining the Raman spectral changes of methemoglobin/total hemoglobin of different ratios, and the Raman intensities of methemoglobin/total hemoglobin of different ratios at 1 586, 1 605 and 1 637 cm-1 were linearly fitted to realize its quantitative detection. The results show that the completely oxidized MetHb can be obtained when the molar ratio of NaNO2 to Hb is 3.5∶1 whose Raman characteristic peaks are at around 499, 1 340, 1 562 and 1 622 cm-1, and that the linear fitting correlation coefficients R2 of the Raman intensities of methemoglobin/total hemoglobin of different ratios at 1 586, 1 605 and 1 637 cm-1 are 0.972 84, 0.997 97 and 0.991 26 respectively, which shows a good linear relationship. This study indicates that the Raman spectrums of MetHb induced by NaNO2 have characteristic differences when compared with normal Hb, that the locations and intensities of Raman characteristic peaks change correspondingly with the alterations of the ratios of methemoglobin/total hemoglobin, and that there are linear correlations between the ratios and their corresponding Raman intensities, which would provide theoretical bases for the clinical Raman spectral detection and quantitative study of methemoglobinemia. |
| Keywords: | Hemoglobin Methemoglobin Raman spectroscopy Sodium nitrite Methemoglobinemia |
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