A Bulk Acoustic Wave Viscosity Sensor for Determination of Lysozyme Based on Lysis of Micrococcus Lysodeikeicus |
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Authors: | Lili Bao Xiaoge Qu Haimei Chen Xiaoli Su Shouzhuo Yao Wanzhi Wei |
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Institution: | (1) College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China, CN |
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Abstract: | A method for determination of lysozyme with a Bulk Acoustic Wave (BAW) viscosity sensor is presented. It is based on the
bacteriolytic action of lysozyme on Micrococcus lysodeikeicus (M. lysodeikeicus) and the response of the sensor to the viscosity and density change of this process. There was a good correlation between
the frequency shift and the concentration of lysozyme in the range 10–100 μg/ml. The content of lysozyme in human saliva was
determined by this method and the results obtained were in good agreement with those from the conventional turbidimetric method.
This method has an advantage over the conventional turbidimetric method in that the amount of sample needed is smaller, the
procedure is simpler and the concentration range of the bacterium suspension which can be used in the detection was extended.
Received September 11, 1998. Revision March 15, 1999. |
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Keywords: | : Bulk Acoustic Wave viscosity sensor lysozyme Micrococcus lysodeikeicus |
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