紫外-可见光谱法研究Fc(COOH)2和BSA相互作用

采用紫外-可见光谱法(UV-Vis)研究Fc(COOH)₂ (λ_max=255 nm)与BSA(λ_max=277.5) 的相互作用。实验结果表明：Fc(COOH)₂在10~190 μmol·L-1范围内吸光度与浓度呈良好的线性关系(r=0.998 4)，BSA在100~1 900 mg·L-1范围内，吸光度与浓度呈良好的线性关系(r=0.999 2)，BSA与Fc(COOH)₂反应后，最大吸收波长移至275 nm。当固定Fc(COOH)₂或BSA的浓度时，Fc(COOH)₂或BSA的吸光度随着BSA或Fc(COOH)₂浓度的增加而增大，说明Fc(COOH)₂与BSA存在分子间的相互作用，主要是由于Fc(COOH)₂和 BSA能形成氢键，分子链增长，吸收的能量增加，导致吸光度增大。同时考察Fc(COOH)₂和 BSA的吸光度随时间的变化，70 μmol·L-1的Fc(COOH)₂与1 900 mg·L-1的BSA反应0.1，24和96 h后，在λ_max=275 nm处的吸光度由1.062分别变为1.045和0.986；当700 mg·L-1的BSA与190 μmol·L-1的Fc(COOH)₂反应0.1，24和96 h后，在λ_max=275 nm处的吸光度由0.813分别变为0.794和0.750。

Interaction Study of Fc(COOH)2 and BSA by UV-Vis Spectroscopy

The interactions between Fc(COOH)₂ (λ_max=255 nm) and BSA (λ_max=277.5 nm) were studied by UV-Vis spectroscopy. The results showed that, there was a good linear relationship between absorbance and concentration for Fc(COOH)₂ in range of 10~190 μmol·L-1 and for BSA in range of 100~1 900 mg·L-1, respectively. After the reaction of Fc(COOH)₂ and BSA , λ_max shifted to 275 nm to show the existing reaction. When the concentration of Fc(COOH)₂ or BSA was fixed, the absorbance of Fc(COOH)₂ or BSA increased with the increase of BSA or Fc(COOH)₂ concentration, which showed the hydrogen bonding form between Fc(COOH)₂ and BSA and the growth of molecular chain, and the increase of absorbance was attributed to the fact that bigger molecule absorbs more energy and release. Meanwhile, the stability of new formed molecule bond was affected by reaction time, after reacted for 0.1 to 24 and 96 h, the absorbance at 275 nm changed from 1.062 to 1.045 and 0.986 for Fc(COOH)₂ (70 μmol·L-1) and BSA (1 900 mg·L-1), and the absorbance changed from 0.813 to 0.794 and 0.750 for Fc(COOH)₂ (190 μmol·L-1) and BSA (700 mg·L-1), respectively.