激光光源-在线荧光光谱检测中的光强度比较研究

基于激光光源和氙灯光源在线荧光光谱法测定罗丹明B、维生素B₂、荧光素和异硫氰酸荧光素含量比较研究激光光源产生的荧光强度和氙灯光源产生的荧光强度。罗丹明B、维生素B₂、荧光素和异硫氰酸荧光素浓度均为10 μg·mL-1，积分时间100 ms，测定3次得其平均值。在线荧光光谱法最大吸收波长分别为580，450，488和510 nm；最大发射波长依次为594，530，525和524 nm。紫外-可见分光光度法测得其最大吸收波长为557，441，481和490 nm；荧光分光光度法测得其最大发射波长为586，520，519和520 nm。通过测定药物，发现激光光源产生的荧光光强度较强于氙灯光源产生的荧光光强度，原因不仅跟光源有关，而且与药物分子的共轭体系大小、共轭大π键的共平面性及其刚性程度、分子母体上取代基的种类有关，分子所处的外界环境如温度、溶剂、溶液酸碱度、激发光的照射等因素也会影响荧光效率。激光光源和氙灯光源产生的荧光光强度大小顺序为罗丹明B>荧光素>异硫氰酸荧光素>维生素B₂。激光光源在线荧光光谱法在一定程度上填补了在线荧光光谱仪在食品、药品痕量检测方面应用的空白。

Study Comparative Light Intensity of Laser Light Source-Online Fluorescence Spectrum Detection

This paper Used laser light sources and xenon lamp light source of online fluorescence spectrometry to determine the optical content of rhodamine B, vitamin B₂, fluorescein and fluorescein isothiocyanate, and compared the light intensity of laser light sources and xenon lamp light source. Methods:The optical concentration of Rhodamine B, vitamin B₂, fluorescein and different hydrogen sulfate fluorescein were equally 10 μg·mL-1; Integration time was 100 ms, and the average value was measured by three times detection. Maximum absorption wavelength determinated by online fluorescence spectrometry were respectively 580, 450, 488 and 510 nm; The maximum emission wavelength were respectively 594, 530, 525 and 524 nm. The maximum absorption wavelength detemineted by UV spectrophotometry were respectively 557, 441, 481 and 490 nm；Maximum emission wavelength detected by fluorescence method were respectively 586, 520, 519, 520 nm. By measuring medicine, we have found that the laser light source of the fluorescent light intensity is stronger than the xenon lamp light source of the fluorescent light intensity. The reason is not only related to the light source, and with the size of drug molecules conjugated system, large conjugated PI coplanar and rigid degree, the kinds of molecular substituents on the matrix, molecules of the external environment such as temperature, solvent, acid and alkali solution, such factors as the excitation light also can affect the fluorescent efficiency. Laser light sources and xenon lamp light source of the fluorescent light intensity size is rhodamine B> vitamin B₂> fluorescein> fluorescein isothiocyanate in order. Laser light source online fluorescence spectrometry , to a certain extent, fill in the blank of online fluorescence spectrometer application in food, medicine, trace detection.