| IDENTIFICATION OF HUMAN N-ras AS THE COMMON ONCOGENE IN NIH 3T3 CELLS TRANSFORMED WITH DNAs FROM HUMAN PRIMARY HEPATIC CANCER AND HEPATOMA 7402 LINE |
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| 作者姓名： | 顾健人 田培坤 万大方 王翔 李宏年 潘志美 黄乐泓 李秀珍 蒋惠秋 |
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| 作者单位： | Department of Biochemistry and Molecular Biology，Shanghai Cancer Institute，Department of Biochemistry and Molecular Biology，Shanghai Cancer Institute，Department of Biochemistry and Molecular Biology，Shanghai Cancer Institute，Department of Biochemistry and Molecular Biology，Shanghai Cancer Institute，Department of Biochemistry and Molecular Biology，Shanghai Cancer Institute，Department of Biochemistry and Molecular Biology，Shanghai Cancer Institute，Department of Biochemistry and Molecular Biology，Shanghai Cancer Institute，Department of Biochemistry and Molecular Biology，Shanghai Cancer Institute，Department of Biochemistry and Molecular Biology，Shanghai Cancer Institute |
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| 摘 要： | DNA was extracted from NIH 3T3 cells transformed with DNAs from human primary hepatic cancer (PHC) and Hepatoma 7402 cell line. The transformant DNA was analyzed by Southern transfer and hybridization with ~(32)P-labeled probes of various oncogenes.The EcoRI 7.2 and 9.0 kb bands characteristic of human N-ras gene were identified in transformed NIH 3T3 cells derived both from PHC and 7402 DNA. The BamHI 6.6kb band characteristic of human c-Ha-ras I was present only in 7402 transformants, but not in PHC transformants.Using ~(35)S-methionine incorporation, immunoprecipitation with anti-p21 monoclonal antibodies, SDS-PAGE and autoradiography, it was demonstrated that p21 synthesis was remarkably enhanced in 1402 cells as well as in transformed cells derived from both 7402 and PHC DNA.Taking the data together, it. strongly implies that N-ras is one of the transforming genes for human liver cancer.
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