毛细管区带电泳中的pH效应

毛细管电泳是在电场作用下带电粒子在毛细管内的定向移动。溶质在给定时间间隔和给定电场下移动的距离用淌度表示,而淌度受到缓冲液pH的影响显著,因此,确定缓冲液、选择并控制其pH值是毛细管电泳技术的关键。本文对pH值对淌度的影响进行了研究。 1 实验部分 所有实验在HPE-100毛细管电泳仪(Bio-Rad USA)上进行,紫外检测,电动进样。所用毛细管柱中S柱由Bio-Rad提供,H_5、H_6和E均为本实验室自行制备,研制过程和性能考察

The Effect of the Buffer pH in Capillary Zone Electrophoresis

The effect of buffer pH on the migration time of both peptides and proteins was studied in the range from pH 2. 5 to 111 the dependence of the elute order and resolution of the components on the pH is very sensitive and should be optimized. Special attention was also paied to it's effect on the absorption of the wall on the proteins at the basic condition. It is one of the ways to inhibit the absorption, but the pH values should be chosen very carefully.