Genetic diversity of rhizobial populations recovered from three Lotus species cultivated in the infra-arid Tunisian soils |
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Authors: | Mokhtar Rejili Maria Jos Lorite Mosbah Mahdhi Juan Sanjuan Pinilla Ali Ferchichi Mohamed Mars |
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Institution: | aArid & Oases Cropping Laboratory, Arid Area Institute (IRA), Medenine 4119, Tunisia;bSoil Microbiology & Symbiotic Systems Department, Experimental Station of Zaidìn, CSIC, Granada, Spain;cLaboratory of Plant Biotechnology Applied to Crop Improvement, Faculty of Sciences of Gabès, Erriadh City, Zrig 6072, Gabès, Tunisia |
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Abstract: | Eighty-three bacterial strains isolated from root nodules of Lotus creticus, L. pusillus, and L. arabicus grown in infra-arid Tunisian soils were characterized using a polyphasic approach including phenotypic analysis, rep-PCR and PCR-RFLP analyses of the 16S rRNA gene. Phenotypically, all isolates are fast growers the majority of which grow at a pH of between 5.5 and 9. Most of the tested isolates tolerate NaCl concentrations from 1.39% to 3.48%. By rep-PCR fingerprinting, the genomic similarity varied from 30% to 98%. All tested isolates were clustered into 32 rep-PCR clusters at the similarity level of 80%. The genomic divergence of strains revealed by rep/PCR analysis appeared to be very important since a molecular polymorphism delimiting symbionts for each species of Lotus was identified. With the high-resolution of rep-PCR profiles of the isolates obtained using Pearson’s/UPGMA analysis, the isolates were resolved into 60 different profiling groups to undergo 16S ARDRA analyses. The analysis of all restriction fragments from each strain based on the UPGMA algorithm from the combined patterns showed that Lotus isolates are very diverse and that they were affiliated to Sinorhizobium, Rhizobium, and Mesorhizobium genera. |
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Keywords: | Lotus sp rep-PCR fingerprinting PCR-RFLP 16S rRNA Rhizobia Arid habitat |
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