Development of a new biosensor for superoxide radicals |
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Authors: | Email author" target="_blank">Emel?EmregülEmail author |
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Institution: | (1) Department of Chemistry, Faculty of Science, Ankara University, 06100 Ankara, Turkey |
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Abstract: | A superoxide dismutase (SOD) biosensor for determination of superoxide radicals has been developed by immobilization of superoxide
dismutase within gelatin (G) on a Pt electrode surface. The properties of the biosensor have been investigated and optimum
conditions–enzyme concentration, glutaraldehyde concentration, and pH–were determined. The response of the G-SOD biosensor
was proportional to concentration and the detection limit was 0.01 mmol L−1 at a signal-to-noise ratio of 3. The biosensor retained 89% and 60% of its sensitivity after use for three and four weeks,
respectively. Immobilization of SOD on gelatin provides a biocompatible microenvironment around the enzyme and stabilizes
the activity of the enzyme very efficiently. The superoxide dismutase biosensor was used to determine the antioxidant properties
of acetylsalicylic acid-based drugs and the anti-radical activity of healthy and cancerous human brain tissues. |
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Keywords: | Superoxide dismutase Gelatin Biosensor Free radicals Scavengers |
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