葡萄糖酶电极测定环糊精聚合物中的环糊精单元

用多巴胺电氧化聚合物膜将葡萄糖氧化酶固定在铅笔电极上,制得简易的葡萄糖酶电极.用自制的葡萄糖酶电极对自制环糊精聚合物中环糊精单元进行定量检测.研究了自制的葡萄糖酶电极的检测范围和稳定性.结果表明环糊精聚合物中环糊精单元质量与葡萄糖酶电极响应电流间存在很好的线性关系,且酶电极稳定性好,可以多次反复使用,连续使用30天,响应电流只减弱7%.与用Sulfate-Phenol比色法测得的环糊精聚合物中环糊精单元结果相比,两者相对偏差在8%以内.

CONTENT DETERMINATION OF CYCLODEXTRINS IN CYCLODEXTRINS POLYMER BY USING GLUCOSE ENZYMATIC ELECTRODE

An amperometric glucose enzymatic electrode was constructed by using dopamine polymer to immobilize glucose oxidase on the pencil lead.It was used to quantify cyclodextrin units in cyclodextrin polymer.The response to the concentrate of cyclodextrin and the stability of glucose enzymatic electrode were investigated.It was found that a good linear relationship exists between the quantity of cyclodextrin and the response current of the enzymatic electrode.It was also found that the enzyme electrode is very robust and can be used repeatedly.The response current only decreased by 7%,and no obvious deterioration in its response performance was found even if the glucose enzymatic electrode was keep using for one month.Compared with the conventional Sulfate-Phenol method,the relative deviation of the determination results from the two methods in within 8%.