环境胁迫对微小卡罗藻溶血毒素分泌的影响

在实验室条件下,利用溶血实验将培养于不同盐度、pH、温度和光照强度下微小卡罗藻的培养液做溶血检测,探讨不同环境因子胁迫下微小卡罗藻溶血毒素的分泌情况.结果表明：适宜盐度下该藻分泌少量溶血毒素（2hHs30=2.1%）,高盐或低盐胁迫下分泌较多（2hHs50=134.7%,2 hHs20=38.8%）.pH〈7及pH〉9时,分泌的溶血毒素较多（2 hHp5=101.7%,2 hHp10=107.4%）;pH在7～9之间,分泌很少（2 hHp7=1.5%）.高温胁迫能促进溶血毒素的分泌（2 hHt50=70.7%）,适宜温度下不分泌溶血毒素.短时间内（48h）,随着光照强度的增加（0～20000lx）,该藻的生长不受影响,溶血毒素分泌很少.因此,溶血毒素的分泌与该藻的生长环境密切相关,一旦生长受到抑制,毒素分泌量就会明显增大.

Effects of Environmental Stress on Hemolysin Secretion of Karlodinium micrum

The effects of different environmental stress on the Karlodinium micrum are studied by detecting the culture supernatant of the alga,which is cultured under different salinity,pH,temperature and lighting intensity.The results suggest that the Karlodinium micrum can secrete more hemolysin at high(2 hHs50=134.7%) or low(5 hHs20=40.8%) salinity,but less at 30(2 hHs30=2.1%),which is the optimal state for growing.More hemolysin(2 hHp5=101.7%,2 hHp10=107.4%) can be secreted when pH is less than 7 or more than 9,a...