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环境胁迫对微小卡罗藻溶血毒素分泌的影响
引用本文:傅永静,周成旭,严小军.环境胁迫对微小卡罗藻溶血毒素分泌的影响[J].宁波大学学报(理工版),2009,22(2):175-180.
作者姓名:傅永静  周成旭  严小军
作者单位:宁波大学,海洋生物工程重点实验室,浙江,宁波,315211
基金项目:教育部长江学者与创新团队项目 
摘    要:在实验室条件下,利用溶血实验将培养于不同盐度、pH、温度和光照强度下微小卡罗藻的培养液做溶血检测,探讨不同环境因子胁迫下微小卡罗藻溶血毒素的分泌情况.结果表明:适宜盐度下该藻分泌少量溶血毒素(2hHs30=2.1%),高盐或低盐胁迫下分泌较多(2hHs50=134.7%,2 hHs20=38.8%).pH〈7及pH〉9时,分泌的溶血毒素较多(2 hHp5=101.7%,2 hHp10=107.4%);pH在7~9之间,分泌很少(2 hHp7=1.5%).高温胁迫能促进溶血毒素的分泌(2 hHt50=70.7%),适宜温度下不分泌溶血毒素.短时间内(48h),随着光照强度的增加(0~20000lx),该藻的生长不受影响,溶血毒素分泌很少.因此,溶血毒素的分泌与该藻的生长环境密切相关,一旦生长受到抑制,毒素分泌量就会明显增大.

关 键 词:微小卡罗藻  溶血毒素  环境胁迫

Effects of Environmental Stress on Hemolysin Secretion of Karlodinium micrum
FU Yong-jing,ZHOU Cheng-xu,YAN Xiao-jun.Effects of Environmental Stress on Hemolysin Secretion of Karlodinium micrum[J].Journal of Ningbo University(Natural Science and Engineering Edition),2009,22(2):175-180.
Authors:FU Yong-jing  ZHOU Cheng-xu  YAN Xiao-jun
Institution:Key Laboratory of Marine Biotechnology;Ningbo University;Ningbo 315211;China
Abstract:The effects of different environmental stress on the Karlodinium micrum are studied by detecting the culture supernatant of the alga,which is cultured under different salinity,pH,temperature and lighting intensity.The results suggest that the Karlodinium micrum can secrete more hemolysin at high(2 hHs50=134.7%) or low(5 hHs20=40.8%) salinity,but less at 30(2 hHs30=2.1%),which is the optimal state for growing.More hemolysin(2 hHp5=101.7%,2 hHp10=107.4%) can be secreted when pH is less than 7 or more than 9,a...
Keywords:Karlodinium micrum  hemolysin  environmental stress  
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