A FLASH of insight into cellular chemistry: genetically encoded labels for protein visualization in vivo |
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Authors: | Kevin J. Luebke |
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Affiliation: | Address: Center for Biomedical Inventions, Department of Internal Medicine, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75235-8573, USA. |
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Abstract: | Genetically encoded fluorescent labels, such as green fluorescent protein, make it possible to visualize a protein's natural distribution and environment in living cells. A new approach to protein labeling in living cells has been devised in which a small, membrane-permeable ligand binds with high affinity and specificity to a short peptide motif that can be incorporated into the protein of interest; the ligand becomes brightly fluorescent after binding to the peptide. |
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