Determination of sugar alcohols in confectioneries by high-performance liquid chromatography after nitrobenzoylation |
| |
Authors: | Nojiri S Taguchi N Oishi M Suzuki S |
| |
Institution: | Tama Branch Laboratory, Tokyo Metropolitan Research Laboratory of Public Health, Tachikawa, Japan. |
| |
Abstract: | A method was developed for the determination of sugar alcohols, meso-erythritol, xylitol, D-glucitol, D-mannitol, maltitol and parachinit by high-performance liquid chromatography (HPLC). The sugar alcohols were converted into strong ultraviolet (UV)-absorbing derivatives with p-nitrobenzoyl chloride. HPLC was performed on a phenyl column, using acetonitrile-water (67:33) as mobile phase and UV detection (260 nm). The calibration curves for all sugar alcohols tested were linear in the 10-250 microg/ml range. The average recoveries of the sugar alcohols from four sugarless confectioneries spiked at 5 and 10% levels of six sugar alcohol standards ranged from 73.2 to 109.0% with relative standard deviations ranging from 0.7 to 9.0%. The detection limit of the developed method was 0.1% for the above sugar alcohols contained in the samples. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|