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Distinguishing Individual DNA Bases in a Network by Non-Resonant Tip-Enhanced Raman Scattering |
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| Authors: | Dr Rui Zhang Xianbiao Zhang Huifang Wang Dr Yao Zhang Dr Song Jiang Dr Chunrui Hu Dr Yang Zhang Prof Dr Yi Luo Prof Dr Zhenchao Dong |
| Institution: | 1. Hefei National Laboratory for Physical Sciences at the Microscale and Synergetic Innovation Center of Quantum Information and Quantum Physics, University of Science and Technology of China, Hefei, Anhui, 230026 China
Department of Theoretical Chemistry and Biology, School of Biotechnology, Royal Institute of Technology, 10691 Stockholm, Sweden These authors contributed equally to this work.;2. Hefei National Laboratory for Physical Sciences at the Microscale and Synergetic Innovation Center of Quantum Information and Quantum Physics, University of Science and Technology of China, Hefei, Anhui, 230026 China These authors contributed equally to this work.;3. Hefei National Laboratory for Physical Sciences at the Microscale and Synergetic Innovation Center of Quantum Information and Quantum Physics, University of Science and Technology of China, Hefei, Anhui, 230026 China |
| Abstract: | The importance of identifying DNA bases at the single-molecule level is well recognized for many biological applications. Although such identification can be achieved by electrical measurements using special setups, it is still not possible to identify single bases in real space by optical means owing to the diffraction limit. Herein, we demonstrate the outstanding ability of scanning tunneling microscope (STM)-controlled non-resonant tip-enhanced Raman scattering (TERS) to unambiguously distinguish two individual complementary DNA bases (adenine and thymine) with a spatial resolution down to 0.9 nm. The distinct Raman fingerprints identified for the two molecules allow to differentiate in real space individual DNA bases in coupled base pairs. The demonstrated ability of non-resonant Raman scattering with super-high spatial resolution will significantly extend the applicability of TERS, opening up new routes for single-molecule DNA sequencing. |
| Keywords: | DNA-Basen Molekulare Erkennung Nichtresonante Raman-Spektroskopie Rastertunnelmikroskopie Spitzenverstärkte Raman-Streuung |