A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione |
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Authors: | Zhixue Liu Prof. Xin Zhou Yu Miao Ying Hu Nahyun Kwon Prof. Xue Wu Prof. Juyoung Yoon |
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Affiliation: | 1. Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules, Ministry of Education, Research Centre for Chemical Biology, Department of Chemistry, Yanbian University, Yanji, 133-002 China;2. Department of Chemistry and Nano Science, Ewha Womans University, Seoul, 120-750 Korea |
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Abstract: | The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH-related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG- 1 ) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant (Kd) of 2.59 mm and a fast response time (t1/2=5.82 s). We also demonstrate that QG- 1 detection of GSH is feasible in a model protein system. QG- 1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40±0.87 mm ). |
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Keywords: | Antioxidantien Fluoreszenzsonden Gluthionin Ratiometrische Sonden Redoxhomöostase |
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