Co-immobilized Phosphorylated Cofactors and Enzymes as Self-Sufficient Heterogeneous Biocatalysts for Chemical Processes |
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Authors: | Dr. Susana Velasco-Lozano Ana I. Benítez-Mateos Dr. Fernando López-Gallego |
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Affiliation: | Heterogeneous biocatalysis group, CIC biomaGUNE, Edificio Empresarial “C”, Paseo de Miramón 182, 20009 Donostia, Spain |
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Abstract: | Enzyme cofactors play a major role in biocatalysis, as many enzymes require them to catalyze highly valuable reactions in organic synthesis. However, the cofactor recycling is often a hurdle to implement enzymes at the industrial level. The fabrication of heterogeneous biocatalysts co-immobilizing phosphorylated cofactors (PLP, FAD+, and NAD+) and enzymes onto the same solid material is reported to perform chemical reactions without exogeneous addition of cofactors in aqueous media. In these self-sufficient heterogeneous biocatalysts, the immobilized enzymes are catalytically active and the immobilized cofactors catalytically available and retained into the solid phase for several reaction cycles. Finally, we have applied a NAD+-dependent heterogeneous biocatalyst to continuous flow asymmetric reduction of prochiral ketones, thus demonstrating the robustness of this approach for large scale biotransformations. |
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Keywords: | biocatalysis cofactor regeneration heterogenous catalysis porous materials protein immobilization |
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