Two-photon excited fluorescence from biological aerosol particles |
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| Authors: | Vasanthi Sivaprakasam Janet W Lou Jay D Eversole |
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| Institution: | a Optical Sciences Division, Naval Research Laboratory, 4555 Overlook Avenue, S.W. Washington, DC 20375, USA
b Global Defense Technology & Systems, Inc., Mission Systems Division at Naval Research Laboratory, 4555 Overlook Avenue, S.W. Washington, DC 20375, USA |
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| Abstract: | We used a 40 MHz mode-locked 524 nm laser source to evaluate the utility of sub-picosecond excitation of fluorescence from 2-photon absorption in biological aerosols. Individual particles of biological composition, as well as other calibration particles, suspended in an inlet air flow were illuminated and measured as they passed through an optical chamber. To our knowledge, this was the first demonstration of 2-photon excited fluorescence from micron-sized particles composed of micro-organisms. We also observed a high fluorescence signal at visible wavelengths, which was not present with single-photon excitation. |
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| Keywords: | Laser induced fluorescence Particle scattering 2-Photon excitation Aerosol Biological particle |
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