SPR imaging as a tool for detecting mucin – anti-mucin interaction. Outline of the development of a sensor for near-patient testing for mucin |
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Authors: | Alfonso Fernández-González Joanna Rychłowska Rosana Badía Reiner Salzer |
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Institution: | Institut für Analytische Chemie, Technische Universit?t Dresden, 01062, Dresden, Germany On leave from Departamento de Química, Física y Analítica, Universidad de Oviedo. Julián Clavería S.N., Oviedo, Spain
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Abstract: | Sensors able to provide ‘yes’/‘no’ answers have become of interest in recent years, especially in the fields of environmental
research and healthcare. We describe a procedure based on surface plasmon resonance imaging (SPRI) to investigate the interaction
between mucin and anti-mucin antibody, and we outline the development of an alarm sensor for the protein mucin, whose high
concentration in saliva, blood or tissue is related to illnesses such as gingivitis, peridontitis or even cancer. Anti-mucin
gastric antibodies are immobilised onto a gold surface. The immobilisation is evaluated for neat gold chips and for polymer-modified
gold surfaces. We found that two different pHs are required, one for the immobilisation of the antibodies on gold (pH 5.5)
and a different one for optimal interaction between the sample and the antibody layer (pH 7.0). Finally, we briefly demonstrate
the application of the sensor to real saliva samples, both mucin-less and mucin-containing, evaluating the potential of the
sensor to discriminate between healthy and ill. |
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Keywords: | : Biosensors SPRI surface plasmon resonance imaging alarm sensor |
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