| 以金属离子为增稳剂的酶法终点法测定MPT- NAG含量 |
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| 引用本文： | 陶国锋,连国军. 以金属离子为增稳剂的酶法终点法测定MPT- NAG含量[J]. 广东微量元素科学, 2011, 18(10): 40-43 |
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| 作者姓名： | 陶国锋 连国军 |
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| 作者单位： | 上虞市创烨生物有限公司,浙江上虞,312300 |
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| 摘 要： | 建立了快速、简便、灵敏地测定MPT - NAG含量的酶法终点法.以金属离子为增稳剂,N-乙酰-β-D-氨基葡萄糖苷酶(NAG)催化6-甲基-2-硫代吡啶-N-乙酰-β-D-氨基葡萄糖苷(MPT - NAG)水解,生成产物6-甲基-2巯基吡啶(MPT),MPT在340 nm处有吸收峰,通过测定340 nm处吸光度值计算M...
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| 关 键 词： | MPT- NAG MPT N-乙酰-β-D-氨基葡萄糖苷酶 光度分析法 |
A New Enzymatic End Point Assay of MPT - NAG Using Metal Ions as Stabilizer |
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| TAO Guofeng,LIAN Guojun. A New Enzymatic End Point Assay of MPT - NAG Using Metal Ions as Stabilizer[J]. Trace Elements Science, 2011, 18(10): 40-43 |
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| Authors: | TAO Guofeng LIAN Guojun |
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| Affiliation: | (Shangyu Chuangye Bioscience Co Ltd,Zhejiang Shangyu 312300,China) |
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| Abstract: | To establish a rapid,simple,sensitive enzymatic end point assay of MPT-NAG using metal ions as stabilizer,the analysis is based on the fact that the MPT-NAG,when hydrolyzed in the presence of N-acetyl-beta-D-glucosaminidase,liberates MPT as the chromogen.The absorbence of the chromogen is direct ratio to the concentration of MPT-NAG.The result shows that the method is liner in the range of 0~50 mmol/L,the average rate of recovery is 101.5%,the within run and between run CV are 0.010 5~0.026 4 and 0.013 2~0.031 6,respectively.Comparing this method with HPLC,as the regression equation: y=1.021 0 x + 0.882 1,r=0.998 9.This method was simple,sensitive and suitable for application. |
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| Keywords: | MPT-NAG MPT N-acetyl-beta-D-glucosaminidase spectrophotometry |
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