Preparative isolation of hydrolysable tannins chebulagic acid and chebulinic acid from Terminalia chebula by high-speed counter-current chromatography |
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Authors: | Han Quanbin Song Jingzheng Qiao Chunfeng Wong Lina Xu Hongxi |
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Institution: | Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Shatin N. T., Hong Kong, China. |
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Abstract: | As a chromatographic column, the high-speed counter-current chromatography system was equipped with a preparative HPLC series, enabling the successful isolation of hydrolysable tannins from the fruits of Terminalia chebula, a traditional Chinese medicine. The two-phase solvent system was composed of n-hexane-ethyl acetate-methanol-water (1:20:1:20 v/v). As a result, 33.2 mg chebulagic and 15.8 mg chebulinic acids were obtained in one step from 300 mg of crude extract. Their purities were determined by HPLC to be 95.3 and 96.1%, respectively. The chemical structures were identified by their MS and 1H NMR spectra. |
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Keywords: | Chebulagic acid Chebulinic acid Counter‐current chromatography Hydrolyzable tannin Terminalia chebula |
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