Fast HPLC method for the determination of glimepiride, glibenclamide, and related substances using monolithic column and flow program |
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Authors: | El Deeb Sami Schepers Udo Wätzig Hermann |
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Affiliation: | Institute of Pharmaceutical Chemistry, TU Braunschweig, Braunschweig, Germany. |
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Abstract: | This work presents a fast method for the simultaneous separation and determination of glimepiride, glibenclamide, and two related substances by RP LC. The separation was performed on a Chromolith Performance (RP-18e, 100 mm x 4.6 mm) column. As mobile phase, a mixture of phosphate buffer pH 3, 7.4 mM, and ACN (55:45 v/v) was used. Column oven temperature was set to 30 degrees C. The total chromatographic run time was 80 s. This was achieved using a flow program from 5 to 9.9 mL/min. Precisions of the interday and the intraday assay for both retention times and peak areas for the four analyzed compounds were less than 1.2%. The method showed good linearity and recovery. The short analysis time makes the method very valuable for quality control and stability testing of drugs and their pharmaceutical preparations. |
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Keywords: | Fast HPLC Flow programming Glibenclamide Glimepiride Monolithic columns |
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