Tudy on drug displacement interactions by capillary electrophoresis-frontal analysis |
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Authors: | Dawei Zhou Li Famei |
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Institution: | (1) Pharmaceutical analysis department, Tianjin University, Tianjin, 300072, China |
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Abstract: | The interaction between 18-methyl norethindrone and ketoprofen, including the displacement of ketoprofen from human serum
albumin binding sites, was investigated by the capillary electrophoresis-frontal analysis method (CE-FA) at room temperature.
A very large sample plug was introduced hydrostatically into the capillary (65 cm × 50 μm i.d.; effective length of 35 cm)
over 80 s at a height difference of 11 cm. The working conditions for CE-FA separation are as follows: operating voltage,
10 kV; running buffer, 67 mmol·L−1 phosphate, pH 7.4. The unbound ketoprofen concentration was directly measured from the height of the frontal peak. When the
concentration of 18-methyl norethindrone was increased from 0 to 200 μmol/L, the unbound ketoprofen concentration was found
to increase from 22.4 to 26.4 μmol/L at 100 μmol/L total ketoprofen concentration and from 82.1 to 106.2 μmol/L at 200 μmol/L
total ketoprofen concentration. From these data, it may be deduced that the administration of high concentration of 18-methyl
norethindrone can displace ketoprofen from its secondary binding site.
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Translated from Chinese Journal of Chromatography, 2005, 23(2)(in Chinese) |
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Keywords: | capillary electrophoresis-frontal analysis human serum album in displacement interaction |
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