Growth ofschizosaccharomyces pombe on glucose-malte mixtures in continuous cell-recycle cultures kinetics of substrate utilization |
| |
Authors: | Jean-Louis Uribelarrea José Humberto De Queiroz Alain Pareilleux |
| |
Affiliation: | (1) Centre de Bioingénierie Gilbert Durand, Institut National des Sciences Appliquées, Complexe Scientifique de Rangueil, CNRS-UMR 550L, L.A.-INRA, 31077 Toulouse Cedex, France |
| |
Abstract: | The aerobic growth ofSchizosaccharomyces pombe on mixtures of glucose and malate was investigated during continuous high cell density cultures with partial cell-recycle using a membrane bioreactor. Determination of the specific metabolic rates relative to substrates and products allowed the capacity of the yeast to metabolize malic acid under both oxidative metabolism (carbon limited cultures) and oxidofermentative metabolism (carbon sufficient cultures) situations to be characterized. Under carbon limiting conditions, the specific rate of malate utilization was dependent on the residual concentration and a limit for a purely oxidative breakdown without ethanol formation was observed for a characteristic ratio between the rates of substrate consumption qm/qg of 1.63 g.g-1. In addition, the mass balance analysis revealed the incorporation of malic acid into biomass. In carbon excess environments, the specific rate of malate utilization was dependent on both the residual malate and the specific rate of glucose consumption indicating that in addition to its conversion into ethanol malate can be respiratively metabolized for qm/qg ratios higher than 0.4 g.g-1. |
| |
Keywords: | Schizosaccharomyces pombe malate utilization growth kinetics cell-recycle culture |
本文献已被 SpringerLink 等数据库收录! |