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Novel Composite Plastics Containing Silver(I) Acylpyrazolonato Additives Display Potent Antimicrobial Activity by Contact
Authors:Prof. Fabio Marchetti  Dr. Jessica Palmucci  Prof. Claudio Pettinari  Dr. Riccardo Pettinari  Dr. Francesca Condello  Dr. Stefano Ferraro  Dr. Mirko Marangoni  Prof. Alessandra Crispini  Dr.  Stefania Scuri  Prof.  Iolanda Grappasonni  Prof. Mario Cocchioni  Dr. Massimo Nabissi  Dr. Michele R. Chierotti  Prof. Roberto Gobetto
Affiliation:1. School of Science and Technology, Chemistry Section, University of Camerino, Via S. Agostino 1, Camerino (MC) (Italy);2. School of Pharmacy, Chemistry Section, University of Camerino, Via S. Agostino 1, Camerino (MC) (Italy);3. Analisi Control S.r.l. Via San Claudio, 5, 62014 Corridonia (MC) (Italy);4. Centro di Eccellenza CEMIF.CAL‐LASCAMM, CR‐INSTM, Unità della Calabria, Dipartimento di Chimica e Tecnologie Chimiche, University of Calabria, 87030 Arcavacata di Rende (CS) (Italy);5. Dipartimento di Chimica, University of Torino, Via P. Giuria 7, and Centro di Eccellenza NIS, 10125 Torino (Italy)
Abstract:New silver(I) acylpyrazolonato derivatives displaying a mononuclear, polynuclear, or ionic nature, as a function of the ancillary azole ligands used in the synthesis, have been fully characterized by thermal analysis, solution NMR spectroscopy, solid‐state IR and NMR spectroscopies, and X‐ray diffraction techniques. These derivatives have been embedded in polyethylene (PE) matrix, and the antimicrobial activity of the composite materials has been tested against three bacterial strains (E. coli, P. aeruginosa, and S. aureus): Most of the composites show antimicrobial action comparable to PE embedded with AgNO3. Tests by contact and release tests for specific migration of silver from PE composites clearly indicate that, at least in the case of the PE, for composites containing polynuclear silver(I) additives, the antimicrobial action is exerted by contact, without release of silver ions. Moreover, PE composites can be re‐used several times, displaying the same antimicrobial activity. Membrane permeabilization studies and induced reactive oxygen species (ROS) generation tests confirm the disorganization of bacterial cell membranes. The cytotoxic effect, evaluated in CD34+ cells by MTT (3‐(4,5‐dimethylthiazole‐2‐yl)‐2,5‐diphenyltetrazoliumbromide) and CFU (colony forming units) assays, indicates that the PE composites do not induce cytotoxicity in human cells. Studies of ecotoxicity, based on the test of Daphnia magna, confirm tolerability of the PE composites by higher organisms and exclude the release of Ag+ ions in sufficient amounts to affect water environment.
Keywords:antibiotics  antiproliferation  polymers  silver  solid‐state structures
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