Capabilities of polymer-modified monodisperse colloidal silica particles as biomaterial carrier |
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| Authors: | K Yoshinaga K Kondo A Kondo |
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| Institution: | (1) Department of Applied Chemistry Kyushu Institute of Technology Sensui, Tobata, Kitayushu 804, Japan, JP;(2) Department of Chemical Science and Engineering Kobe University 1-1 Rokkoudaichou Nada, Kobe 675, Japan, JP |
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| Abstract: | Polymer modification of monodispersed colloidal silica (0.5 μm) with poly(maleic anhydride-co-styrene) (P(MA-ST)) and poly
(maleic anhydride-co-methyl methacrylate) (P(MA-MMA)) and application of the composite particles to biomaterial carriers were
investigated. The reaction of bovine serum albumin(BSA)-immobilized P(MA-MMA)/SiO2 with the anti-BSA antibody showed higher sensitivity in immunological agglutination test than BSA–P(MA-ST)/SiO2, though immobilization efficiency of BSA on P(MA-MMA)/SiO2 was lower than that on P(MA-ST)/SiO2. Alkaline phosphatase and glucose oxidase immobilized on the composite particles exhibited extremely low activities, but
α-chymotrypsin immobilized on P(MA-MMA)/SiO2 and its derivative particles showed the relative activity of 12.5% and 16.1% to the native enzyme, respectively. Grafting
of a hydrophilic polymer of poly(acrylic acid) to P(MA-ST)/SiO2 let to an increase of the immobilized α-chymotrypsin activity to give the maximum relative activity of 55.5%.
Received: 23 August 1996 Accepted: 16 October 1996 |
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| Keywords: | Biomaterial carrier immunolatex protein immobilization monodisperse colloidal silica polymer modification |
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