Simple Liquid Chromatographic Determination of Minocycline in Brain and Plasma |
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| Authors: | A. Milane C. Fernandez G. Bensimon V. Meininger R. Farinotti |
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| Affiliation: | (1) Department of Clinical Pharmacy, EA 2706, Faculty of Pharmacy, University of Paris XI, Chatenay-Malabry, France;(2) Department of Pharmacy, Pitie Salpetriere Hospital, Assistance Publique-Hopitaux de Paris, Paris, France;(3) Department of Pharmacology, Pitie Salpetriere Hospital, Assistance Publique-Hopitaux de Paris, Paris, France;(4) Department of Neurology, Pitie Salpetriere Hospital, Assistance Publique-Hopitaux de Paris, Paris, France |
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| Abstract: | A new high-performance liquid chromatography assay was developed for the determination of minocycline in plasma and brain. A solid–liquid extraction procedure was coupled with a reversed-phase HPLC system. The system requires a mobile phase consisting of acetonitrile:water:perchloric acid (26:74:0.25, v/v/v) adjusted to pH 2.5 with 5 M sodium hydroxide for elution through a RP8 column (250 × 3.0 mm, i.d.) with UV detection set at 350 nm. The method proved to be accurate, precise (RSD < 20%) and linear between 0.15–20 μg mL−1 in plasma and 1–20 μg mg−1 in brain. The method was successfully applied to a blood-brain barrier minocycline transport study. |
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| Keywords: | Column liquid chromatography Tetracycline antibiotics Minocycline in blood and brain |
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