| Crystallographic Studies of Cephalosporin Acylase from Pseudomonas sp. Strain 130 |
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| 作者姓名： | 丁怡 姜卫红 张淑平 茅翔 Mark Bartlam 赵国平 饶子和 |
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| 作者单位： | [1]LaboratoryofStructuralBiologyandLaboratoryofProteinScienceoftheMinistryofEducation,DepartmentofBiologicalSciencesandBiotechnology,TsinghuaUniversity,Beijing100084,China [2]ShanghaiInstituteofPlantPhysiology,ChineseAcademyofSciences,Shanghai20000,China [3]Towhomcorrespondenceshouldbeaddressed.,ChineseAcademyofSciences,Shanghai20000,China |
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| 基金项目： | Supported by the National Natural Science Foundation (No. 39970 15 5 )，the High- Technology Developm ent
Program of China (No. 2 0 0 1AA2 330 11)，and the
National Frontier Research Program (No.
G19990 75 6 0 2，G19990 1190 2 and19980 5 110 5 ) |
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| 摘 要： | The cephalosporin acylases are a group of enzymes that hydrolyze cephalosporin C and/or glutaryl 7-aminocephalosporanic acid to produce ?-aminocephalosporanic acid. The cephalosporin acylase from Pseudomonas sp. strain 130 was crystallized in two different forms suitable for structural studies. A tetragonal crystal form diffracted to 0.24 nm belonged to the space group P41212. There was one αβ heterodimer per asymmetric unit. A second crystal form diffracted to 0.21nm belonged to the space group P21. There was four αβ heterodimers per asymmetric unit. The tetragonal crystal structure of CA-130 was determined using the multiwavelength anomalous diffraction method and the P21 crystal structure was then determined using the molecular replacement method.
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| 关 键 词： | 头孢菌素酰基转移酶 水解酶 假单胞菌 结晶学 硒代蛋氨酸 |
Crystallographic Studies of Cephalosporin Acylase from Pseudomonas sp. Strain 130 |
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| Mark Bartlam.Crystallographic Studies of Cephalosporin Acylase from Pseudomonas sp. Strain 130[J].Tsinghua Science and Technology,2003,8(4):395-401. |
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| Authors: | Mark Bartlam |
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| Abstract: | The cephalosporin acylases are a group of enzymes that hydrolyze cephalosporin C and/or glutaryl 7-aminocephalosporanic acid to produce 7-aminocephalosporanic acid.The cephalosporin acylase from Pseudomonas sp.strain 130 was crystallized in two different forms suitable for structural studies.A tetragonal crystal form diffracted to 0.24 nm belonged to the space group P41212.There was one αβ heterodimer per asymmetric unit.A second crystal form diffracted to 0.21 nm belonged to the space group P21.There was four αβ heterodimers per asymmetric unit.The tetragonal crystal structure of CA-130 was determined using the multiwavelength anomalous diffraction method and the P21 crystal structure was then determined using the molecular replacement method. |
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| Keywords: | cephalosporin acylase crystallization selenomethionine (Se Met) multiwavelength anomalous diffraction (MAD) heterodimer heterotetramer |
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