| Miniaturized Cell Lysis Device Using Spherically Focused Ultrasound |
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| 引用本文： | 李刚,肖宏,郭旻,程京. Miniaturized Cell Lysis Device Using Spherically Focused Ultrasound[J]. 清华大学学报, 2003, 8(4): 487-492,497 |
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| 作者姓名： | 李刚 肖宏 郭旻 程京 |
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| 作者单位： | [1]NationalEngineeringResearchCenterforBeijingBiochipTechnology,Beijing100084,China [2]DepartmentofBiologicalSciencesandBiotechnology,TsinghuaUniversity,Beijing100084,China |
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| 基金项目： | Supported by the National Science Foundation for Outstanding Young Scientists of China(No.3982 5 10 8) |
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| 摘 要： | A prototype of a miniaturized cell lysis device developed using a concave spherical transducer is capable of lysing bacteria without added chemical denaturants, enzymes or microparticles and is capable ofefficiently lysing yeast without any mechanical or enzymatic pretreatment. The device is designed for miniature bio-analysis systems where cell lysing is needed to obtain intracellular materials for further analysis such as DNA identification. The device lysis efficiency was evaluated using viable cell counts and microscopy.Additionally, the device efficiency was compared with that of traditional chemical cell lysis methods using standard molecular biological techniques such as agarose gels and ultraviolet (UV) spectroscopy. The results indicate that efficient bacteria and cell disruption can be achieved through a low-voltage-driven and spherically focused high-frequency ultrasonic device.
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| 关 键 词： | 细胞溶菌作用 小型化 球状聚集超频率音响 紫外线光谱法 |
Miniaturized Cell Lysis Device Using Spherically Focused Ultrasound |
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| LI Gang ,,XIAO Hong ,,GUO Min ,CHENG Jing . Miniaturized Cell Lysis Device Using Spherically Focused Ultrasound[J]. Tsinghua Science and Technology, 2003, 8(4): 487-492,497 |
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| Authors: | LI Gang XIAO Hong GUO Min CHENG Jing |
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| Affiliation: | LI Gang 1,2,XIAO Hong 1,2,GUO Min 2,CHENG Jing 1,2** 1. Department of Biological Sciences and Biotechnology,Tsinghua University,Beijing 100084,China, 2. National Engineering Research Center for Beijing Biochip Technology,Beijing 100084,China |
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| Abstract: | A prototype of a miniaturized cell lysis device developed using a concave spherical transducer is capable of lysing bacteria without added chemical denaturants, enzymes or microparticles and is capable of efficiently lysing yeast without any mechanical or enzymatic pretreatment. The device is designed for miniature bio analysis systems where cell lysing is needed to obtain intracellular materials for further analysis such as DNA identification. The device lysis efficiency was evaluated using viable cell counts and microscopy. Additionally, the device efficiency was compared with that of traditional chemical cell lysis methods using standard molecular biological techniques such as agarose gels and ultraviolet (UV) spectroscopy. The results indicate that efficient bacteria and cell disruption can be achieved through a low voltage driven and spherically focused high frequency ultrasonic device. |
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| Keywords: | cell lysis spherically focused ultrasound miniaturization sample pre preparation |
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