野生蕈菌孢子的傅里叶变换红外光谱研究

傅里叶变换红外光谱法(FTIR)研究了9种野生蕈菌的孢子,分析了主要吸收峰的归属,结果显示孢子的主要成分为脂类物质、蛋白质和多糖等。在1800—750cm-1光谱范围内,不同属、不同种的野生蕈菌孢子光谱显示了一定的差异,可以用来区分不同种类的孢子。对1800—1500cm-1和1200—1000cm-1范围的二阶导数光谱分别进行相关分析,9种孢子在1800—1500cm-1范围内的相关性相对较好,相关系数在0.843—0.998范围,表明它们含有的主要化学成分脂类物质、蛋白质等比较相似;在1200—1000cm-1范围内相关性较差,相关系数最大为0.780,最小为0.144,表明多糖物质构型或含量具有较大差异。傅里叶变换红外光谱结合特征峰分析和相关分析极有可能用于鉴别蕈菌孢子。

FTIR Spectroscopic Study of Spores of Wild Growing Mushrooms

Fourier transform infrared spectroscopy(FTIR)was used to study spores of wild growing mushrooms.The infrared spectra of spores are mainly composed of the absorption of lipids,protein and polysaccharide.The spectra exhibit small differences in the range of 1800-750cm~(-1).The second derivative spectra in two regions of 1800-1500cm~(-1) and 1200-1000cm~(-1) were used to evaluate correlation coefficients between the spectra.The spores of different species of wild growing mushrooms have good correlation in the spectra range of 1800-1500cm~(-1),their correlation coefficients vary from 0.843 to 0.998,which indicate that the main chemical components of spores such as lipids and protein are very similar.The correlation coefficients vary from 0.144 to 0.780 in the range of 1200-1000cm~(-1),which indicate the great differences in the structure and contents of polysaccharide between different species spores.The FTIR spectra combined with correlation analysis might be used to identify spores of mushrooms rapidly and conveniently.