| 高效液相色谱法测定茶叶中的茶氨酸 |
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| 引用本文： | 朱小兰,陈波,罗旭彪,姚守拙. 高效液相色谱法测定茶叶中的茶氨酸[J]. 色谱, 2003, 21(4): 400-402 |
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| 作者姓名： | 朱小兰 陈波 罗旭彪 姚守拙 |
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| 作者单位： | 湖南师范大学化学化工学院,湖南,长沙,410081 |
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| 基金项目： | 十五"国家科技攻关项目,湖南省科技厅重点资助(2001BA746C),湖南省自然科学基金(02JJY2023)项目. |
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| 摘 要： | 建立了未衍生化高效液相色谱法(HPLC)测定茶叶中茶氨酸含量的方法。采用的色谱条件为:C 18 色谱柱,以0.05%(体积分数)三氟乙酸水溶液为流动相,流速1 mL/min ,进样量10 μL,检测波长203 nm。茶氨酸质量浓度在0.02～1 g/L 内,其浓度与峰面积呈良好的线性关系,最低检出限为1.75 ng(S/N=3),回收率为97.2%,相对标准偏差(RSD)为1.7%。同时以高效液相色谱-电喷雾离子化质谱对所分离的茶氨酸进行了纯度鉴定。方法具有精确、灵敏、流动相组成简单等特点。
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| 关 键 词： | 茶氨酸 茶叶 高效液相色谱法 高效液相色谱-质谱 |
| 文章编号： | 1000-8713(2003)04-0400-03 |
| 修稿时间： | 2002-09-16 |
Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography |
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| ZHU Xiaolan,CHEN Bo,LUO Xubiao,YAO Shouzhuo. Determination of Theanine in Tea by Reversed-Phase High Performance Liquid Chromatography[J]. Chinese journal of chromatography, 2003, 21(4): 400-402 |
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| Authors: | ZHU Xiaolan CHEN Bo LUO Xubiao YAO Shouzhuo |
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| Abstract: | High performance liquid chromatography (HPLC) coupled wiht electro-spray ioniza tion mass spectrometry (ESI-MS) was used for the determination and identificat ion of theanine in tea without derivatization. A reversed-phase HPLC method was developed and the effects of the mobile phase composition on separation were investigated. Theanine can be separated completely from other components on a Sp herigel C 18 column with trifluoroacetic acid solution (pH 3 0) as mobile phase. The UV detection wavelength was set at 203 nm. The limit of detection wa s down to 1 75 ng. The method is simple and accurate. |
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| Keywords: | high performance liquid chromatography liquid chromatography-mass spectrometry theanine tea |
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