Key Conditions in Capillary Electrophoresis of Amino Acids with Indirect Ultraviolet Detection |
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Authors: | Zhi Xin WANG Yi CHEN Qing GUO |
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Institution: | Zhi Xin WANG; Yi CHEN;Qing GUO Center for Molecular Science. Institute of Chemistry. The Chincse Academy of Sciences. Beijing 100080 |
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Abstract: | Key factors for capillary electrophoresis of amino acids with indirect ultraviolet detection were investigated. Buffer compositions and additives were found to be influential as expected, while buffer PH was shown to be particularly critical to tile separation and detection. The effect of PH also depended on the electrophoretic mode used. Ways to obtain recurring and selective separations were suggested. |
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Keywords: | Amino acids capillary electrophoresis indirect ultraviolet detection |
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