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Key Conditions in Capillary Electrophoresis of Amino Acids with Indirect Ultraviolet Detection
Authors:Zhi Xin WANG  Yi CHEN  Qing GUO
Institution:Zhi Xin WANG; Yi CHEN;Qing GUO Center for Molecular Science. Institute of Chemistry. The Chincse Academy of Sciences. Beijing 100080
Abstract:Key factors for capillary electrophoresis of amino acids with indirect ultraviolet detection were investigated. Buffer compositions and additives were found to be influential as expected, while buffer PH was shown to be particularly critical to tile separation and detection. The effect of PH also depended on the electrophoretic mode used. Ways to obtain recurring and selective separations were suggested.
Keywords:Amino acids  capillary electrophoresis  indirect ultraviolet detection
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