| 大孔吸附树脂分离纯化仙人掌中总黄酮的研究 |
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| 引用本文： | 韦国锋,黄祖良,何有成. 大孔吸附树脂分离纯化仙人掌中总黄酮的研究[J]. 离子交换与吸附, 2010, 26(1) |
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| 作者姓名： | 韦国锋 黄祖良 何有成 |
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| 作者单位： | 右江民族医学院应用化学研究室,百色,533000 |
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| 基金项目： | 广西自然科学基金资助课题(桂科基2360747) |
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| 摘 要： | 考察了4种大孔吸附树脂对仙人掌总黄酮的吸附分离性能,筛选出效果最佳的树脂为AB-8。以总黄酮的吸附量、总黄酮含量和回收率为考察指标,采用紫外分光光度法测定总黄酮。确定了AB-8树脂吸附分离仙人掌总黄酮的工艺条件:上样浓度为15mg/mL,仙人掌总黄酮最大吸附量为18.6mg/mL,吸附流速为5mL/min,洗脱剂为70%乙醇,洗脱剂用量为6倍柱体积,树脂可重复使用3次。经AB-8树脂分离纯化后,总黄酮含量从29%提高到76%,总黄酮回收率为86%。实验结果表明,AB-8树脂可用于仙人掌总黄酮的分离纯化。
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| 关 键 词： | 仙人掌 总黄酮 大孔吸附树脂 吸附分离 |
STUDY ON THE SEPARATION AND PURIFICATION OF TOTAL FLAVONE IN CACTUS BY MACROPOROUS ADSORPTION RESINS |
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| WEI Guofeng,HUANG Zuliang,HE Youcheng. STUDY ON THE SEPARATION AND PURIFICATION OF TOTAL FLAVONE IN CACTUS BY MACROPOROUS ADSORPTION RESINS[J]. Ion Exchange and Adsorption, 2010, 26(1) |
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| Authors: | WEI Guofeng HUANG Zuliang HE Youcheng |
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| Affiliation: | WEI Guofeng HUANG Zuliang HE Youcheng Department of Applied Chemistry,Youjiang Medical College for Nationalities Guangxi Baise 533000,China |
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| Abstract: | To assess the capability of 4 kinds of macroporus adsorption resins for absorbing and separating total flavone in cactus.The optimum adsorption results were got by AB-8 resin.The evaluation indexes included total flavone adsorption capacity,total flavone content,and recovery rate.The UV spectrophotometric method was used in determination of total flavone.The technological condition of adsorption and separating total flavone in opuntia dillenii Haw:the extract concentration was 15mg/mL,the maximum adsorption... |
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| Keywords: | Cactus Total flavone Macroporous adsorption resin Adsorption and separation. |
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