Capillary-assembled microchip as an on-line deproteinization device for capillary electrophoresis |
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Authors: | Hideaki Hisamoto Seigi Takeda Shigeru Terabe |
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Institution: | (1) Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan |
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Abstract: | A capillary-assembled microchip (CAs-CHIP), prepared by simply embedding square capillaries in a lattice polydimethylsiloxane
(PDMS) channel plate with the same channel dimensions as the outer dimensions of the square capillaries, has been used as
a diffusion-based pretreatment attachment in capillary electrophoresis (CE). Because the CAs-CHIPs employ square-section channels,
diffusion-based separation of small molecules from sample solutions containing proteins is possible by using the multilayer
flow formed in the square section channel. When a solution containing high-molecular-weight and low-molecular-weight species
makes contact with a buffer solution, the low-molecular-weight species, which have larger diffusion coefficients than the
high-molecular-weight species, can be collected in a buffer-solution phase. The collected solution containing the low-molecular-weight
species is introduced into the separation capillary to be analyzed by CE. This type of system can be used for CE analysis
in which pretreatment is required to remove proteins. In this work a fluorescently labeled protein and rhodamine-based molecules
were chosen as model species and a feasibility study was performed.
![MediaObjects/216_2006_331_Figb_HTML.gif](/content/xp8741jh54733t84/MediaObjects/216_2006_331_Figb_HTML.gif) |
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Keywords: | Capillary-assembled microchip Capillary electrophoresis Deproteinization Polydimethylsiloxane Square capillary |
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