十六烷基溴化吡啶与脱氧核糖核酸作用的共振光散射光谱及其分析应用

在碱性条件下，十六烷基溴化吡啶(CPB)与脱氧核糖核酸(DNA)共存时，体系产生较强的共振光散射，其强度与DNA浓度呈线性关系，据此提出了基于阳离子表面活性剂的共振光散射法定量测定DNA。在最佳实验条件下，测得小牛胸腺DNA(ctDNA)和鱼精子DNA(fsDNA)的线性范围分别为0．2-2．0mg／L和0．2—1．25mg／L，检出限分别为0．07mg／L和0．05mg／L。该方法已应用于合成样品及实际样品中DNA含量的测定。

Resonance Light Scattering Spectra of Cetylpyridinium Bromide and Deoxyribonucleic Acid System and Its Application to Deoxyribonucleic Acid Assay

A cationic surfactant, cetylpyridinium bromide (CPB) was used to determine deoxyribonucleic acids (DNA) at nanogram level with a resonance light scattering (RLS) technique. The electrostatic interactions between CPB and DNA gave rise to three characteristic peaks of RLS at 263 nm, 363 nm and 492 nm. Under optimal conditions, the enhanced intensity of RLS was proportional to the concentrations of DNA in the range of 0.2~2.0 mg/L for calf thymus DNA and 0.2~1.15 mg/L for fish sperm DNA. The corresponding limits of detection were 0.07 mg/L and 0.05 mg/L, respectively. The relative standard deviation of ten replicate measurements was 2.2% for 0.75 mg/L fish sperm DNA. This method was used to detect DNA in ~synthetic and real samples successfully.