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Biological activity of functionalized SiO2 thin films prepared by sol-gel method |
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| Authors: | Roberta Collino Joël Therasse Frédéric Chaput Jean-Pierre Boilot Yves Levy |
| Institution: | (1) Laboratoire d'Immunologie et de Biochimie, Centre d'Etudes du Bouchet, BP 3-91710, Vert-le-Petit, France;(2) Groupe de Chimie du Solide, Laboratoire de Physique de la Matière Condensée, Ecole Polytechnique, CNRS URA, D 1254-91128 Palaiseau, France;(3) Laboratoire d'Optique Non Linéaire, Institut d'Optique Théorique et Appliquée, Bat. 503, BP 147-91403 Orsay Cedex, France |
| Abstract: | In this work we investigated the biological properties of sol-gel films in aqueous medium. Functionalized silica films were prepared by the sol-gel process, from organically modified silicon alkoxides with amino or thiol groups. Covalent binding of proteins with different orientations according to the hydrophilic or hydrophobic character of the surface was studied. This binding occurred via a covalent coupling agent providing a very stable linkage. No denaturation was detected and a good detection of the antigen was observed. Immunoassays have demonstrated the biological activity of grafted antibodies. |
| Keywords: | sol-gel functionalized films grafting of antibodies biological activity |
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