A method to evaluate the relative weights of structural and functional constraints on a genome |
| |
Authors: | C Cosmi M F Macchiato A Tramontano |
| |
Institution: | (1) Dipartimento di Scienze Fisiche, Università di Napoli, Napoli, Italia;(2) European Molecular Biology Laboratory, Heidelberg, RFG |
| |
Abstract: | Summary A method that numerically evaluates the relative influence of structural or functional constraints on the codon composition
of a gene is presented. The method applies to any constraint provided that it can be translated intoa priori probability table of codon usage. The method is very easy to apply to different constraints and to different pools of sequences.
Theoretical codon usage obtained from a linear combination of the various constraints was compared with experimental codon
frequencies of several classes of genes taxonomically homogeneous and, applying a minimization tecnique, the relative weights
of the examined constraints have been evaluated. The results show that a common strategy exists for all coding sequences even
though most of the constraints are represented by different probability tables for different pools of genes. We also demonstrate
that the method is reliable when it is applied to a single gene, so that it can also be used as a tool to predict the characteristics
of a gene from its sequence. |
| |
Keywords: | General theoretical and mathematical biophysics (including logic of biosystems quantum biology relevant aspects of thermodynamics information theory cybernetics and bionics) |
本文献已被 SpringerLink 等数据库收录! |